Ethanol production from dilute-acid pretreated rice straw by simultaneous saccharification and fermentation with Mucor indicus, Rhizopus oryzae, and Saccharomyces cerevisiae S W Article
Karimi K., Emtiazi G., Taherzadeh M.J.
Enzyme and Microbial Technology Volume 40, Issue 1, 2006 , Pages 138-144
(IF=2.502 ,,JCR2016) (CiteScore=2.83 ,Quartile 1,Scopus2016)
Abstract:
Ethanol production from rice straw by simultaneous saccharification and fermentation (SSF) with Mucor indicus, Rhizopus oryzae, and Saccharomyces cerevisiae was investigated and compared with pure cellulose, Avicel, as a reference. The straw was pretreated with dilute-acid hydrolysis. The SSF experiments were carried out aerobically and anaerobically at 38 °C, 50 g/l dry matter (DM) solid substrate concentration and 15 or 30 filter paper unit (FPU)/g DM of a commercial cellulase. The experiments were ended after 7 days, while an average of 2-3 days were usually enough to achieve the maximum ethanol yield. All the strains were able to produce ethanol from the pretreated rice straw with an overall yield of 40-74% of the maximum theoretical SSF yield, based on the glucan available in the solid substrate. R. oryzae had the best ethanol yield as 74% from rice straw followed by M. indicus with an overall yield of 68% with 15 FPU/g DM of cellulase. Glycerol was the main byproduct of the SSF by M. indicus and S. cerevisiae with yields 117 and 90 mg/g of equivalent glucose in the pretreated straw, respectively, while R. oryzae produced lactic acid as the major byproduct with yield 60 mg/g glucose equivalent in pretreated rice straw under anaerobic conditions. © 2006 Elsevier Inc. All rights reserved.
Keywords :
Dilute-acid hydrolysis; Ethanol; Mucor indicus; Rhizopus oryzae; Rice straw; Saccharomyces cerevisiae; Simultaneous saccharification and fermentation